electric cell-substrate impedance sensing (ecis) device  (Applied BioPhysics)

Journal: Scientific Reports

Article Title: Exploring the heterogeneity in glioblastoma cellular mechanics using in-vitro assays and atomic force microscopy

doi: 10.1038/s41598-025-04841-4

Figure Lengend Snippet: Normalized resistance showing migration of U87 MG and T98G cells over ECIS electrodes for 168 hours (7 days): ( a ) Three independent experiments, N1, N2, and N3 for each cell type, involving tens of thousands of cells over the electrodes. ( b ) Average of normalized resistance for the three experiments of ( a ) for each cell type. ( c ) Independent t-test showing statistically significant (***** representing \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$P<0.00005$$\end{document} ) difference between the migration of the two cell lines. ( d ) Typical phase contrast microscopy images of T98G and U87 MG cells on day 3 of the experiment (68 hours) showed markedly different morphologies. Number of cells measured: 4000.

Article Snippet: The commercially available Electric Cell-Substrate Impedance Sensing (ECIS) device (Applied Biophysics, New York, NY, USA) is a non-invasive, robust device for electrically measuring a variety of cell characteristics, including morphology, proliferation, and migration , .

Techniques: Migration, Microscopy

Journal: Biosensors

Article Title: Electric Cell-Substrate Impedance Sensing (ECIS) as a Platform for Evaluating Barrier-Function Susceptibility and Damage from Pulmonary Atelectrauma

doi: 10.3390/bios12060390

Figure Lengend Snippet: Schematic of ( a ) the ECIS Flow Device, ( b ) Node with 10 electrodes, and ( c ) setup of the cell culture system and the RD experiment. ( a ) The ECIS flow device has a single straight channel with length × width × depth = 50 mm × 5 mm × 0.36 mm. ( b ) Node 1 to 8 (left to right) are placed along the axial direction, covering approximately 70% of the bottom where the H441 cell layer is cultured. Each node has 10 electrodes, having a 250 µm diameter. ( c ) Growth media fills the channel from the reservoir during the culturing of a confluent monolayer that covers the electrodes. Recruitment is induced by a penetrating finger of air that is driven by a computer-controlled actuator pump. A reverse motion reintroduces liquid to complete one RD cycle. The device is tilted at a 45° angle to avoid bubble break-up during the RD motion.

Article Snippet: We monitored the morphological response of a cultured H441 monolayer to the RD stimulus using impedance spectroscopy with an Electrical Cell-Substrate Impedance Sensing (ECIS) device (Applied Biophysics, Troy, NY, USA), based on Giaever and Keese (1991) [ ].

Techniques: Cell Culture

Journal: Biosensors

Article Title: Electric Cell-Substrate Impedance Sensing (ECIS) as a Platform for Evaluating Barrier-Function Susceptibility and Damage from Pulmonary Atelectrauma

doi: 10.3390/bios12060390

Figure Lengend Snippet: Schematics of ECIS electrode configuration and AC current flow paths (not to scale, based on [ , ]). ( a ) The node consists of 10 electrodes, each of which has a diameter of 250 µm providing the surface area to be covered with 100–1000 cells. ( b ) At relatively low frequencies (<2 kHz) most of the current flows under and between adjacent cells (through tight junctions), and at higher frequencies (>20 kHz) more current passes directly through the insulating cell membranes . ( c ) An explanatory simplified electrical circuit to demonstrate the impedance modeling parameters in the current flows through and around cells. The modeling parameters of basal adhesion function ( α ) and intercellular barrier function ( Rb ) are represented as resistors, since both parameters are dependent on the binding and the electrical pathway gap. Cell membrane capacitance ( Cm ) is dependent on the composition of the cell membrane. Ce is the electrode capacitance and Rf is the resistance of the growth media. In the current study, we use resistance at frequency 1 kHz, R (1K), to monitor the formation of cell–cell tight junction, and capacitance at 64 kHz, C (64K), to monitor the formation of confluent cell layer on electrodes.

Article Snippet: We monitored the morphological response of a cultured H441 monolayer to the RD stimulus using impedance spectroscopy with an Electrical Cell-Substrate Impedance Sensing (ECIS) device (Applied Biophysics, Troy, NY, USA), based on Giaever and Keese (1991) [ ].

Techniques: Binding Assay, Membrane